. Author manuscript; available in PMC: 2018 Mar 29.

Published in final edited form as: Microbiology (Reading). 2008 Sep;154(Pt 9):2659–2667. doi: 10.1099/mic.0.2008/019877-0

Table 1.

Primers used for site-directed mutagenesis of AzoA

Underlined nucleotides indicate mutations incorporated into primers.

Mutation	Primer sequences (5′→3′)
W105Y	GCTAATCCAATGTACAACTTAAACGTAC
W105Y	GTACGTTTAAGTTGTACATTGGATTAGC
W105Q	GTAATTGCTAATCCAATGCAAAACTTAAACGTACCGAC
W105Q	GTCGGTACGTTTAAGTTTTGCATTGGATTAGCAATTAC
W105F	GTAATTGCTAATCCAATGTTCAACTTAAACGTACCGAC
W105F	GTCGGTACGTTTAAGTTGAACATTGGATTAGCAATTAC
W105H	GTAATTGCTAATCCAATGCACAACTTAAACGTACCG
W105H	CGGTACGTTTAAGTTGTGCATTGGATTAGCAATTAC
W105A	GTAATTGCTAATCCAATGGCGAACTTAAACGTACCG
W105A	CGGTACGTTTAAGTTCGCCATTGGATTAGCAATTAC
W105G	GCTAATCCAATGGGAAACTTAAACGTAC
W105G	GTACGTTTAAGTTTCCCATTGGATTAGC