. Author manuscript; available in PMC: 2018 Mar 29.

Published in final edited form as: Microbiology (Reading). 2005 May;151(Pt 5):1433–1441. doi: 10.1099/mic.0.27805-0

Table 1.

Purification of recombinant Azo1 of S. aureus expressed in E. coli Activities were determined with Methyl Red as substrate.

Step	Total protein (mg)	Total activity (U)	Specific activity [U (mg protein)⁻¹]	Yield (%)
Crude cell extract	549	8·32	0·015	100
HiPrep Q XL	167	5·68	0·034	68
HiPrep Phenyl FF	76	4·33	0·057	52
Mono Q	34	2·75	0·081	33
HiLoad Superdex 200	12	1·68	0·14	20