. Author manuscript; available in PMC: 2018 Mar 29.

Published in final edited form as: Microbiology (Reading). 2005 May;151(Pt 5):1433–1441. doi: 10.1099/mic.0.27805-0

Table 2.

Effects of enzyme stages and cofactors on the azoreductase activity of S. aureus Azo1

Enzyme stages	Cofactors added	Specific activity [U (mg protein)⁻¹]
Original enzyme^*	NADPH	0·15
Original enzyme	NADH	ND^†
Original enzyme	NADPH+FMN	0·14
Reconstructed with FMN^‡	NADPH	0·12
Apoprotein	NADPH+FMN	0·13
Apoprotein	NADPH+FAD	ND
Apoprotein	NADPH+riboflavin	ND

Original enzyme, the purified intact recombinant S. aureus Azo1.

^†

ND, not detectable.

^‡

Reconstructed with FMN, the apoprotein reconstructed with FMN on the column.