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Effect of FFA challenge on lipid accumulation: HepG2 cells were loaded with FFA mixtures (250 and 500 μM) for 20 hours. Nile red and Hoechst-33342 staining (magnification 40×) (A), Nile red fluorescence intensity (B), and ORO colorimetric assay (C). The values are expressed as mean± SEM from six independent replicates. FFAs, free fatty acids; ORO, Oil Red O; SEM, standard errors of mean. * P<0.05; ***P<0.001.
