Table 1.
Gene-special primers used for real-time polymerase chain reaction
| Gene | Pathway | Sequence (5' → 3') | Gene Bank no./ref |
|---|---|---|---|
| Human FATP5 | Fatty acid uptake | Forward: TGATGGGACTTGTCGTTGG | NM_012254 |
| Reverse: CCAGAAGCAGGAAGTAGAGAAC | |||
| Human SCD | Lipid synthesis | Forward: AGTTCTACACCTGGCTTGG | NM_005063 |
| Reverse: GTTGGCAATGATCAGAAAGAGC | |||
| Human PPARα | Fatty acid oxidation | Forward: CAATGCACTGGAACTGGATGA | NM_001001928 |
| Reverse: GTTGCTCTGCAGGTGGAGTCT | |||
| Human CPT1 | Fatty acid oxidation | Forward: TCCAACTCACATTCAGGCAG | NM_001031847.2 |
| Reverse: TTAAACATCCGCTCCCACTG | |||
| Human FOXO1 | Gluconeogenesis | Forward: CTACGAGTGGATGGTCAAGAG | NM_002015 |
| Reverse: ATGAACTTGCTGTGTAGGGAC | |||
| Human PEPCK | Gluconeogenesis | Forward: GAGAATACTGCCACACTGACC | NM_001018073 |
| Reverse: CCGCTGAGAAGGAGTTACAATC | |||
| Human β-actin | Housekeeping gene | Forward: GTCTTCCCCTCCATCGT | NM_007393.5 |
| Reverse: CGTCCCCACATGGAAT |
FATP5, fatty acid transport protein 5; SCD, stearoyl CoA desaturase; PPARα, peroxisome proliferator activated receptor α; CPT1, carnitine palmitoyltransferase 1; FOXO1, forkhead box protein O1; PEPCK, phosphoenolpyruvate carboxykinase.