Table 2. Mutations differentiating Clostridium difficile RT017 imipenem-resistant isolates found at hospital A from imipenem-susceptible isolates found at hospital B, Portugal.
| Gene in M68 genome* | Genome position* | Nucleotide in M68 | Nucleotide change† | Amino acid change† | Gene product |
|---|---|---|---|---|---|
| RS02665 | 512416 | C | C578T | Ala193Val‡ | Multidrug ATP-binding cassette transporter permease, associated with antimicrobial drug resistance |
| RS04280/pbp1 | 905394 | G | G1663A | Ala555Thr‡ | Penicillin-binding transpeptidase |
| RS04935 | 1048151 | C | T1010C | Ile337Thr‡ | 3-Isopropylmalate dehydratase large subunit |
| RS05670/pbp3 | 1221182 | G | A2162C | Tyr721Ser‡ | Penicillin-binding protein |
| RS07765 | 1666351 | G | G214T | Gly72§ | Hypothetical protein |
| RS07795/hisB | 1671129 | T | T209C | Ile70Thr‡ | Imidazoleglycerol-phosphate dehydratase |
| RS07810 | 1673280 | T | C474T | Ala158Ala | Imidazoleglycerol-phosphate synthase cyclase subunit |
| RS08415 | 1792079 | G | A241G | Lys81Glu‡ | Hypothetical protein (domain of MerR-like transcriptional regulators) |
| RS08810 | 1882950 | C | C420T | Asp140Asp | Flavodoxin |
| RS14235 | 3083548 | G | G421T | Gly141§ | Haloacid dehalogenase |
| RS18530 | 4054525 | C | C220T | Gln74§ | S-adenosyl methionine–dependent methyltransferase |
| RS19130/gyrA | 4174650 | C | C245T | Thr82Ile‡ | DNA gyrase subunit A |
| RS19545 | 4255124 | C | C400T | His134Tyr‡ | Phage portal, SPP1 Gp6-like family protein |
*Relative to the annotation of the C. difficile M68 genome (GenBank accession no. NC_017175). †Changes observed between imipenem-resistant and imipenem-susceptible isolates. ‡Nonsynonymous mutations. §Mutations leading to putative protein truncation.