Table 2. Titers of influenza A(H7N9) GD virus in organs of experimentally infected mice*.
| Virus |
Postinfection day 3, mean log10 PFU ± SD/g |
|
Postinfection day 6, mean log10 PFU ± SD/g | ||||
|---|---|---|---|---|---|---|---|
| Lung |
NT |
Brain |
Lung |
NT |
Brain |
||
| GD wild-type | 5.7 ± 0.2 | 4.2 ± 0.8 | ND | 5.8 ± 0.4 | 5.9 ± 0.7 | 3.2 | |
| GD/PB2-482K | ND, p<0.01 | ND, p<0.01 | ND | 3.0, 3.4, p<0.01 | 2.6 ± 0.4, p<0.05 | ND | |
| GD/PB2-588A | 3.3 ± 0.6, p<0.01 | 3.1, p<0.01 | ND | 4.6 ± 0.8 | 4.4 ± 2.5 | ND | |
| GD/PB2-482K-588A | ND, p<0.01 | ND, p<0.01 | ND | 3.1 ± 0.3, p<0.05 | ND, p<0.01 | ND | |
| GD/PA-497K | 5.1 ± 0.2, p<0.05 | 2.9 ± 1.0 | ND | 4.6 ± 1.0 | 5.8 ± 0.4 | ND | |
| GD/PB2-482K-588A+PA-497K | ND, p<0.01 | ND, p<0.01 | ND | 2.2, 2.7, p<0.01 | ND, p<0.01 | ND | |
*BALB/c mice were intranasally inoculated with 102 PFU of virus (in 50 μL). Three animals per group were euthanized on postinfection days 3 and 6. Statistically significant differences compared with GD wild-type–infected mice were determined by use of a 1-way analysis of variance followed by a Dunnett test. GD, A/Guangdong/17SF003/2016; ND, virus not detected (detection limit ≈2 log10 PFU/g); NT, nasal turbinates; PA, polymerase acidic; PB, polymerase basic.