Figure 3.

Increased FoxM1B expression in regenerating liver of old tg mice stimulates expression of cell cycle-promotion genes. RNase protection assay demonstrates increased expression of cell cycle-promotion genes in regenerating liver of old tg mice. Total RNA was prepared from regenerating livers from 2-month-old wt mice (A), 12-month-old tg mice (B), and 12-month-old wt mice (C) at indicated hours after PHx (numbers along the top). The liver RNA samples were used for RNase protection assays (in triplicate) to determine expression of the indicated cyclin genes as well as the Cdc25B and p55Cdc genes as described in Materials and Methods. Note that representative duplicate samples are shown.