Figure 4.

Footprints left behind at chromosomal sites after Minos excision. DNA was extracted from thymus and spleen of a double-transgenic mouse (Upper) or from an embryonic fibroblast cell line from a MCG/+ mouse after transfection with a transposase-expressing plasmid (Lower) and used as template for PCR with the flanking primers. PCR-amplified bands were cloned, and 32 clones (19 from thymus and spleen and 13 from fibroblast cells) were sequenced. TA is the target-site duplication. Nucleotides in red correspond to the ends of the transposon terminal repeats; nucleotides in blue are of unknown origin. The flanking nucleotides and TA repeats are aligned.