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. 2018 Mar 26;11:1721–1732. doi: 10.2147/OTT.S157715

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© 2018 Bao et al. This work is published and licensed by Dove Medical Press Limited

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Down-regulation of miR-1269 inhibited the growth and promoted the apoptosis in A549 cells. (A) RT-PCR was used for determining the miR-1269 expressions after A549 cell transfection with miR-1269 inhibitors. (B) Cell Counting Kit-8 (CCK8) was used for determining cell viability after A549 cell transfection with miR-1269 inhibitors. (C and D) Colony formation assay was used for determining cell proliferation ability. (E and F) Flow cytometry analysis showed cell apoptosis after A549 cell transfection with miR-1269 inhibitors; U6 was detected as the control of sample loading. Data are expressed as the mean ± SD for three independent experiments. **P<0.01 versus control group.