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. 2018 Mar 26;11:1721–1732. doi: 10.2147/OTT.S157715

Figure 4.

Figure 4

miR-1269 regulated tumor protein p53 (TP53) and caspase-9 expressions and caspase-3 activity. (A) Putative targets predicted by TargetScan. (B) Relative luciferase activity resulted from binding of TP53 3′-untranslated region (UTR) reporter and miR-1269 in A549 cell 48 h after transfection. (C) Putative targets predicted by TargetScan. (D) Relative luciferase activity resulted from binding of caspase-9 3′-UTR reporter and miR-1269 in A549 cell 48 h after transfection. (E) RT-PCR was used for detecting TP53 and caspase-9 expressions after A549 cell transfection with miR-1269 mimics. (F and G) Western blot was used for detecting TP53 and caspase-9 expressions after A549 cell transfection with miR-1269 mimics. (H) Caspase-3 activity detection assay was used for detecting caspase-3 activity in miR-1269 mimics group. (I) RT-PCR was used for detecting TP53 and caspase-9 expressions after A549 cell transfection with miR-1269 inhibitors. (J and K) Western blot was used for detecting TP53 and caspase-9 expressions after A549 cell transfection with miR-1269 inhibitors. (L) Caspase-3 activity detection assay was used for detecting caspase-3 activity in miR-1269 inhibitors. Tubulin was detected as the control of sample loading. Data are expressed as the mean ± SD for three independent experiments. *P<0.05 and **P<0.01 versus control+TP53/or control group.