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. 2018 Mar 26;11:1721–1732. doi: 10.2147/OTT.S157715

Figure 5.

Figure 5

TP53/or caspase-9 silencing regulated the viability and apoptosis in A459 cells. (A) RT-PCR was used for determining TP53 expressions after A459 cell transfection with TP53-siRNA. (B) Western blot was used for determining TP53 expressions after A459 cell transfection with TP53-siRNA. (C) CCK8 was used for determining cell viability after A459 cell transfection with TP53-siRNA. (D and E) Flow cytometry analysis showed cell apoptosis after A549 cell transfection with TP53-siRNA. (F) RT-PCR was used for determining caspase-9 expressions after A459 cell transfection with caspase-9-siRNA. (G) Western blot was used for determining caspase-9 expressions after A459 cell transfection with caspase-9-siRNA. (H) CCK8 was used for determining cell viability after A459 cell transfection with caspase-9-siRNA. (I and J) Flow cytometry analysis showed cell apoptosis after A549 cell transfection with caspase-9-siRNA. Tubulin was detected as the control of sample loading. Data are expressed as the mean ± SD for three independent experiments. **P<0.01 versus siNC group.

Abbreviations: CCK8, Cell Counting Kit-8; siCaspase-9, cell transfection with caspase-9-siRNA; siNC, cell transfection with NC; siTP53, cell transfection with TP53-siRNA; TP53, tumor protein p53.