Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 26;11:65–84. doi: 10.2147/DMSO.S159315

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Singh et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

Effect of Fst overexpression on AdipoQ/FGF21/pAMPK pathway and FGF21 target genes. Western blot analysis of key proteins in Epi and SC adipose tissues from WT and Fst-Tg mice (A), and recombinant Fst protein (0.5 µg/ml) or Fst-enriched supernatant treatment of differentiated 3T3-L1 cells (B) using 100 µg of total tissue or cell lysates. Real-time quantitative gene expression analysis of FGF21, its receptors (Fgfr1-r3) and target genes in Epi (C), and SC (D) adipose tissues obtained from 10-week old WT and Fst-Tg mice. Data are expressed as mean ± SD. *P≤0.05, **P≤0.01, and ***P≤0.001 (n=3).

Abbreviations: WT, wild type; Fst-Tg, follistatin transgenic; DM, differentiation medium; AdipoQ, adiponectin; FGF, fibroblast growth factor; AMPK, AMP-activated protein kinase; PGC, peroxisome proliferator-activated receptor-gamma coactivator; SirT1, sirtuin1; GADPH, glyceraldehyde 3-phosphate dehydrogenase; Epi, epididymal; SC, subcutaneous.