Fig. 1.

Lipidomic analysis of myelin lipids isolated from MS and control NAWM by LC–MS/MS and HPTLC. a Samples were analyzed by LC–MS/MS to obtain quantitative and qualitative information on multiple lipid classes including phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylserine (PS), and phospatidic acid (PA), sphingomyelin (SM), ceramide (Cer), phosphatidylinositol (PI), and free fatty acids (FFA). Lipidomics data obtained through LC−MS/MS from NAWM from 9 MS and 9 control samples show a significant decrease in sphingomyelin (SM) lipids in MS myelin. b Representative HPTLC image shows bands for cholesterol, galactocerebrosides (GalC), and sulfatides visualized by copper acetate spray and heating. Lipid standards for GalC and sulfatide were run in the first lane. The mobility of the sulfatide standard is altered slightly compared to the myelin lipids isolated from the brain tissue due to the absence of cholesterol and other lipids. c Quantitation shows that average sulfatide levels were found to be significantly reduced in MS (n = 9) compared to control samples (n = 9). Error bars represent SEM. *p ≤ 0.05