The concentration of a-ketoglutarate and levels of histone H3 methylation are regulated by NAA. a The concentration of α-ketoglutarate was measured by HILIC–mass spectrometry in control cultured oligodendrocytes, cultures treated with 2 and 20 μM NAA. b Representative Western blots for H3K4me3, H3K9me3, H3K9me2, acetylated histone H3 (Ac-H3), and histone H3 from control oligodendrocyte primary cultures and cultures treated with either 2 or 20 μM NAA. c
Bar diagram showing the densitometry analysis for H3K4me3/H3K9me3/H3K9me2/Ac-H3 normalized to H3 levels. Error bars represent SEM. *p ≤ 0.05, **p ≤ 0.01