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. 2018 Mar 21;9:567. doi: 10.3389/fimmu.2018.00567

Figure 3.

Figure 3

AIRE-mediated recruitment of Dectin receptors to fungal synapse (FS). (A) Representative confocal micrographs of wild-type and AIRE-knockdown macrophage-like THP-1 cells stimulated with C. albicans hyphae for 20 or 30 min to observe the recruitment of Dectin-1 (blue) and Dectin-2 (red) receptors to the FS. (B–E) Each dot represents a cell used for the measurement indicated. The horizontal bars denote the mean. *p < 0.05 as determined by the t-test. (B,C) Percentages of total Dectin-1 (B) and Dectin-2 (C) at the FS compared with that in the whole cell. (D,E) Amounts of Dectin-1 (D) and Dectin-2 (E) at the FS (conjugated-unconjugated cells). (F,G) Synaptic co-localization of Dectin-1 with Dectin-2 (F) or Dectin-2 with Dectin-1 (G). Representative confocal micrographs of 30 cells counted in 3 independent experiments. Scale bar = 5 µm.