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. 2018 Mar 29;6(6):e13654. doi: 10.14814/phy2.13654

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© 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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UMOD effects on interstitial myofibroblasts in UUO nephropathy. The intensity of the interstitial myofibroblast infiltration, evaluated by α SMA western immunoblotting (n = 5 for sham groups; n = 7 for UUO groups), revealed differences in the kinetics of the response, with significantly lower levels on day 7 and higher levels on day 21 in the UMOD +/+ mice (A, B). Representative immunofluorescence photomicrographs illustrate the differences in α SMA staining (red) on day 21, with DAPI nuclear staining in blue (C). Bar graphs represent means +1SEM; * indicates P value < 0.05. Photomicrograph magnification: ×40. Scale bar: 25 μm.