Table 1.

KIM‐1 protein distribution pattern

KIM‐1 Kidney‐to‐Urine Relative Protein Ratiosa
	Sham	Day 7 UUO	Day 14 UUO	Day 21 UUO
UMOD+/+ groups	1.000	0.003	0.159	26.233
UMOD−/− groups	1.066	0.105	4.085	128.619
Kidney‐to‐urine relative ratios for UMOD−/− versus UMOD+/+ groups	1	35	26	5

^aKIM‐1 protein levels in kidney tissue lysates were measured by western blotting and results were corrected for protein loading by measuring GAPDH levels. Final results were expressed in arbitrary units using the mean level in the UMOD+/+ sham group as 1.0 densitometric unit (a). KIM‐1 urinary levels were measured by western blotting and results were corrected to urinary creatinine levels measured in the same samples using the Creatinine Parameter Assay Kit. Final results were expressed in arbitrary units using the mean level in the sham UMOD+/+ group as 1.0 densitometric unit (b). Within each group, all protein or all urine samples (n = 7) were loaded into a single gel, as shown in Figure 3. The data shown is in the first and second rows are the ratios for the mean values of (a) divided by the mean value of (b) at each time‐point. The data in the final row summarizes the relatively higher expression of KIM‐1 protein in the kidney of the UMOD−/− mice (calculated data in second row ÷ first row).