Figure 6.

Images of coronal sections through rostral striatum labeled using in situ hybridization histochemistry, showing that striatal PPE mRNA levels but not PPT mRNA levels were reduced in R6/2 mice. Images of film autoradiograms for pairs of R6/2 and wild‐type littermate mice at 3.5, 6, 8, and 12, weeks of age are shown. A large PPE mRNA reduction is evident in R6/2 mice at 6‐12 weeks of age. No PPT mRNA changes in R6/2 mice are obvious at any age. This illustration is Figure 1 from Sun et al.91 Note that the autoradiographic analyses in this study were performed using radioactivity standards that allowed us to quantify the changes in PPE and PPT expression with age. This analysis showed PPE signal in WT declined from 3.5 to 6 weeks, but somewhat rebounded thereafter. In the case of PPT, signal was less at 6 and 8 weeks than at 3.5 weeks, but rebounded slightly at 12 weeks—see Figure 2 in Sun et al91