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. 2018 Mar 29;13(3):e0194164. doi: 10.1371/journal.pone.0194164

Fig 3. Determination of specific carbonylated proteins in MCF-12A, MDA-MB-231, and MDA-MB-468 cell lines by two-color western.

Fig 3

Representative two-color western blots to detect carbonylation in (A) filamin A, (B) EPRS, and (C) HSP90β proteins using anti-filamin A, anti-EPRS and anti-HSP90β antibodies respectively in combination with anti-DNP antibody. Protein carbonyls were detected using anti-goat 800CW (green), and specific protein bands were detected using donkey anti-rabbit or anti-mouse 680LT (red) as the secondary antibodies. 12A = MCF-12A, 231 = MDA-MB-231, 468 = MDA-MB-468.