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. 2018 Mar 29;13(3):e0193239. doi: 10.1371/journal.pone.0193239

Fig 9. MeV-2 is delivered by M. euphorbiae (Me) into plant tissues during feeding but does not persist in the plant in the absence of the aphid.

Fig 9

(A) Nucleic acids isolated from leaves of naïve tomato plants or from plants infested for 2 weeks with MeV-2-infected M. euphorbiae were used in RT-PCR. (B, C) M. euphorbiae heavily infested tomato leaves were cleared of the aphids. Leaflets were cut into halves longitudinally through the midrib and the detached half was processed for MeV-2 (B) or MeV-3 (C) detection. The second half of the leaflet was left attached to the plant, free of aphids, for 14 additional days before processing. SlUbi3 was used as a positive control. M = molecular weight marker.