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. 2018 Mar 29;13(3):e0195278. doi: 10.1371/journal.pone.0195278

Fig 5. WS19T-mediated mDia2 loss is recoverable upon washout.

Fig 5

A. MDA-MB-231 cells were incubated in DMEM with no media change (DMEM), WS19T-CM (CM), or DMEM with a media refresh of DMEM (D/D) for 8h. Cells in WS19T-CM were then released into DMEM after the 8h incubation. Western blots were performed on cell lysates for the indicated proteins. B. Densitometry was performed on A using Image J with mDia2 expression normalized to GAPDH and compared to DMEM. *p<0.03 relative to DMEM. The experiment was performed in quadruplicate.