Fig 7. mDia2 functional inhibition does not affect mDia2 protein expression.

A. MDA-MB-231 cells were grown to 60% confluency and treated with vehicle (DMSO) or 40μM SMIFH2 for 1-16h prior to lysate collection and Western blotting. B. Densitometry from A. where mDia2 was normalized to GAPDH and compared relative to DMEM. C. MDA-MB-231 cells were treated with 40μM SMIFH2 starting at T0 continuously for 16h during a wound closure experiment. Experiments were repeated thrice.