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. 2018 Mar 29;13(3):e0195278. doi: 10.1371/journal.pone.0195278

Fig 10. CXCL12 mediates MDA-MB-231 mDia2 downregulation while cell migration.

Fig 10

A. HMF, WS21T, and WS19T fibroblasts were plated and CM collected concurrently for each replicate. HMF-CM, WS19T-CM, and WS21T-CM from three independent collections were applied in triplicate to a CXCL12/SDF1α ELISA assay. The experiment was repeated three times. CXCL12 levels were averaged for each CM and compared to HMF-CM. *p<0.0008 HMF-CM relative to WS21T-CM, WS21T-CM relative to WS19T-CM, and HMF-CM relative to WS19T-CM. p<0.001. B. MDA-MB-231 cells were treated with 15, 25, and 100ng/ml CXCL12 and wound closure assays performed for 16h. The assay performed in triplicate and repeated three times. *p<0.001 C. MDA-MB-231 cells were treated with 100ng/ml CXCL12 for 8-72h and cell lysates were Western blotted. mDia2 expression was normalized to GAPDH and compared to the DMEM control. The experiment was repeated three times. *p<0.01 D. MDA-MB-231 cells were treated with WS19T-CM as indicated. Cells were pretreated (P) for 15m with AMD3100, and/or simultaneously and continuously (C) with AMD3100 and CM (CM) or DMEM (DM) for 16h. Cell lysates were Western blotted as indicated.