Figure 3. Hypoxia treatment regulated the expression of SIRT1 through miR-448. A, Expression of miR-448 in AGE1.HN and PC12 cells in control or treated with hypoxia, or transfected with NC or miR-448 inhibitor was determined by qRT-PCR. B, Expressions of SIRT1 mRNA and protein in AGE1.HN and PC12 cells in control or treated with hypoxia or transfected with NC or miR-448 inhibitor were analyzed by qRT-PCR and western blot, respectively. Data are reported as means±SD. *P<0.05 vs control. #P<0.05 vs NC (t-test). NC: negative control.
