Figure 5. Expression of SIRT1 was regulated by miR-448 in AGE1.HN cells. A, Predicted binding region of miR-448 and the 3′-UTR of WT-SIRT1, and sequence of the mut-SIRT1 3′-UTR. B, Relative luciferase activity of WT-SIRT1 and mut-SIRT1 in AGE1.HN cells transfected with NC or miR-448 inhibitor. Expressions of SIRT1 mRNA and protein in AGE1.HN cells transfected with NC or miR-448 inhibitor were analyzed by qRT-PCR and western blot, respectively. *P<0.05 vs NC (t-test). C, Relative luciferase activity of WT-SIRT1 and mut-SIRT1 in AGE1.HN cells transfected with pre-NC or miR-448 mimic. Expressions of SIRT1 mRNA and protein in AGE1.HN cells transfected with pre-NC or miR-448 mimic were analyzed by qRT-PCR and western blot, respectively. Data are reported as means±SD. #P<0.05 vs pre-NC (t-test). NC: negative control.
