Table 1.

Selectivity of BM3h variants for Ir(Me)-DPIX with respect to heme in minimal and rich media^a

Variant	M9 media Ir(Me)-DPIX : hemeb	TB media Ir(Me)-DPIX : hemeb	M9 yield Ir(Me)-DPIX (mg/L)c
BM3h-T268A	95.0:5.0 ± 3.2	37.8:62.2 ± 3.6	6.8
BM3h-T268A/C400A	98.4:1.6 ± 2.2	80.2:19.8 ± 2.8	3.5
BM3h-T268A/C400G	99.5:0.5 ± 0.5	81.9:18.1 ± 6.2	4.1
WIVS-FM*-T268A	96.7:3.3 ± 2.1	51.8:48.2 ± 9.6	9.4
WIVS-FM*-T268A/C400A	99.7:0.3 ± 0.5	97.2:2.8 ± 1.1	11.5
WIVS-FM*-T268A/C400G	>99.9:n.d. ± n/a	98.3:1.7 ± 1.5	14.3

^aAll values reported are the average and standard deviations from three independent experiments.

^bDetermined by HPLC analysis of purified proteins from expressions in the denoted media, reported as molar ratios.

^cYields were determined via HPLC analysis or from absorbance spectra using ε_{402 nm} = 107.7 mM⁻¹cm⁻¹