Table 2.

Activities and stereoselectivities of biocatalysts for the reaction of styrene with ethyl diazoacetate.^a

catalyst	yield [%]	TTNb	dr (cis:trans)	ee cis [%]c	ee trans [%]d
heme	16	80	13:87	0	−3
Ir(Me)-DPIX	40	200	29:71	−1	−4
BM3h-T268A/ heme	68	339	1:99	−11	−97
WIVS-FM*-T268A/C400G/Ir(Me)-DPIX	48	240	29:71	0	0

^areaction conditions: 7.5 mM olefin, 10 mM EDA, 15 μM catalyst, in 0.1 M NaP_I pH 6.0 100 mM NaCl buffer, 5-% DMF as cosolvent (reactions with heme and heme enzymes also contain 10 mM dithionite). TTN and stereoselectivities determined by chiral GC analysis.

^bTTN = total turnover number.

^c(1S, 2R) – (1R, 2S).

^d(1R, 2R) – (1S, 2S).