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. 2018 Jan 25;29(4):1154–1164. doi: 10.1681/ASN.2017060675

Figure 3.

Figure 3.

IDH2 increases cellular respiration in MPTCs. (A) Respiration was analyzed using a Seahorse. Seven micromolar carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) and 6 μM Rotenone and Antimycin A (Rot/AA) were added to determine (B) baseline, (C) maximal, and (D) complex 2 OCR. (E) Maximal respiration minus baseline respiration was used to calculate SRC (n=9 wells per group). All bar graphs are expressed as mean±SD. *P<0.05, IDH2 versus control; **P<0.05, IDH2 versus mock; #P<0.05, myc Idh2 versus control; $P<0.05, myc Idh2 to mock.