Figure 1.

Deletion of 11 bp in the NBCe1-A specific 5` coding sequence causes a downstream frameshift mutation that results in an early stop codon. Top panel shows results of DNA sequencing of NBCe1-A KO mice and WT mice and bottom panel shows the translated amino acid sequence. NBCe1-A KO mice have an 11 bp deletion beginning at nucleotide 128 in the coding sequence for the NBCe1-A gene. This results in a three amino acid deletion in the translated protein followed by a frameshift mutation that results in an early stop codon at amino acid 53. Sequence alignment performed using ClustlX, v 2.1. * indicates an identical nucleotide/amino acid. “Stop” indicates termination of translation because of a stop codon in the mRNA sequence.