No colocalization is observed between HCN3+ and cKIT+ utPMCs and tdTomato-labeled renal stroma, tailbud mesenchyme, metanephric mesenchyme, or ureteric bud. (A and B) TOMATO-labeled Foxd1-Cre+ renal stromal cells (red color, A and B) do not colocalize with (A) HCN3 or (B) cKIT (green color). (C and D) TOMATO-labeled Tbx18-Cre+ renal tailbud mesenchyme cells (red color, C and D) do not colocalize with (C) HCN3 or (D) cKIT (green color). (E and F) TOMATO-labeled Rarb2-Cre+ metanephric mesenchyme cells (red color, E and F) do not colocalize with (E) HCN3 or (F) cKIT (green color). (G and H) TOMATO-labeled Hoxb7-Cre+ ureteric bud cells (red color, G and H) do not colocalize with (G) HCN3 or (H) cKIT (green color). cKIT, KIT Proto-Oncogene Receptor Tyrosine Kinase; DAPI, 4′,6-Diamidino-2-Phenylindole; HCN3, Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 3; utPMCs, urinary tract pacemaker cells.