Table 1.

MID calculation obtained from labeled A549 cell extract diluted in plasma (1:1000, 1:500, 1:200, 1:100, and 1:10) of the original ¹³C enrichment) with model peak correction and without model peak correction; determination of accuracy (3 injections) and reproducibility (standard deviation, 3 extractions) of minimal MI abundance, slope and R² determined by regression analysis.

Metabolite	Concentration Range (µM)	MI	Model Peak					No Model Peak
			Min MI Abundance (%)	Accuracy (n = 3)	Reproducibility (n = 3)/Stdev	Slope	R2	Min MI Abundance (%)	Accuracy (n = 3)	Reproducibility (n = 3)/Stdev	Slope	R2
Serine	87.9–96.2	M3	0.0207	0.0263	0.0054	0.76	0.98	0.5800	0.0728	0.0400	0.27	0.81
Alanine	302.0–330.0	M3	0.0165	0.0059	0.0089	0.82	0.99	0.8950	0.0565	0.1293	0.18	0.65
Lactate	1540–1620	M3	0.0054	0.0022	0.0013	1.02	1	0.0410	0.0062	0.0031	0.97	1
Erythronate	15.0–16.0	M4	0.2317	no data	0.1545	1.01	0.99	4.7448	1.0821	0.3720	0.48	0.82
Fumarate	2.1–2.3	M4	0.4698	2.4931	0.1227	0.9	0.92	5.2324	0.3983	1.8185	0.59	0.96
Glutamine	409.8–469.3	M5	0.0260	0.0022	0.0135	0.95	1	0.3162	0.1518	0.1856	1.22	1
Glucose	6330–6631	M6	0.2337	no data	0.0167	0.6	0.98	0.8812	no data	0.0471	0.34	0.9
Glutamate	540.1–657.0	M5	0.0293	0.0015	0.0038	1	1	0.1024	0.0094	0.0095	0.98	1
Succinate	9.6–10.4	M4	0.1001	0.0831	0.0639	0.73	0.95	1.8842	0.1708	0.3157	0.3	0.95