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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Arthritis Rheumatol. 2018 Mar 7;70(4):566–577. doi: 10.1002/art.40399

FIGURE 5. Blocking TIM-3 in MLR cultures promotes the production of soluble factors altering SSc fibroblast gene expression.

FIGURE 5

SSc fibroblasts were cultured for 24 hrs with supernatants from MLR cultures in Fig. 3. Fibroblast RNA was prepared and expression of selected genes measured with Nanostring. (A) PLSDA was applied to Nanostring data and plot shows LV1 for gene expression data of fibroblasts exposed to supernatants from each anti-co-IR antibody-treated MLR. Data points represent values for each individual healthy and SSc donor. (B) Bar graph shows the contribution of individual genes to LV1. Asterisks designate genes that contributed most to LV1 separation of anti-TIM-3-mediated changes, as calculated by VIP score.