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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Stem Cells. 2017 Dec 25;36(4):616–625. doi: 10.1002/stem.2752

Figure 1.

Figure 1

A. Immunophenotypic profile of iPSC colonies. Following viral transfection of fibroblasts and culture × 2 to 3 weeks, iPSC colonies stained positive for pluripotency markers OCT3/4, Sox-2, NANOG, TRA-1-60 and TRA-1-81. DAPI stains the nuclei. The colonies were also stained for isotype control antibodies IgG2b (Oct3/4), IgG (Sox-2 and Nanog), and IgM (TRA-1-81 and TRA-1-60). Bar=50μm. B. iPSC colonies imaged at different magnifications (upper 2 panels) show abundant expression of αKlotho (red), counterstained for filamentous actin (Alexa Fluor 488 phalloidin, green) and nuclei (DAPI, blue) Bar = 50 μm (left) and 20 μm (right). Z-section (left lower panel) shows the cuboidal nature of cells and further magnified (right lower panel) to show αKlotho in the cytoplasm. Bar = 20 μm (left) and 10 μm (right).