Table 4.
Correlations between sputum levels of antibodies to cit proteins/peptides and sputum NET levels in At-Risk subjects
| DNA-MPO | DNA-NE | |||
|---|---|---|---|---|
| Cit-Proteins** | r-value | p-value* | r-value | p-value* |
| Apolipoprotein A1 | 0.86 | <0.001 | 0.66 | <0.001 |
| Apolipoprotein E§ | 0.93 | <0.001 | 0.73 | <0.001 |
| Histone 2A | 0.80 | <0.001 | 0.69 | <0.001 |
| Histone 2B | 0.79 | <0.001 | 0.66 | <0.001 |
| Fibrinogen§ | 0.91 | <0.001 | 0.74 | <0.001 |
| Fibronectin | 0.90 | <0.001 | 0.72 | <0.001 |
| Vimentin | 0.79 | <0.001 | 0.67 | <0.001 |
| Cit-Peptides** | ||||
| Apolipoprotein A1 231–248§ | 0.79 | <0.001 | 0.72 | <0.001 |
| Apolipoprotein E 277–296 cyclic§ | 0.89 | <0.001 | 0.80 | <0.001 |
| Biglycan 247–266 cyclic | 0.76 | <0.001 | 0.66 | <0.001 |
| Clusterin 221–240 cyclic | 0.81 | <0.001 | 0.65 | <0.001 |
| Clusterin 231–250 cyclic | 0.74 | <0.001 | 0.67 | <0.001 |
| Histone 2A/a-2 1–20§ | 0.78 | <0.001 | 0.70 | <0.001 |
| Histone 2A/a-2 1–20 cyclic§ | 0.75 | <0.001 | 0.70 | <0.001 |
| Histone 2B/a 62–81 cyclic | 0.80 | <0.001 | 0.68 | <0.001 |
| Enolase 1A 5–21§ | 0.91 | <0.001 | 0.77 | <0.001 |
| Fibrinogen A 27–43§ | 0.83 | <0.001 | 0.74 | <0.001 |
| Fibrinogen A 41–60 cyclic | 0.72 | <0.001 | 0.62 | 0.001 |
| Fibrinogen A 211–230 cyclic§ | 0.80 | <0.001 | 0.69 | <0.001 |
| Fibrinogen A 556–575 cyclic | 0.65 | <0.001 | 0.56 | 0.004 |
| Fibrinogen A 582–599§ | 0.81 | <0.001 | 0.71 | <0.001 |
| Fibrinogen A 616–635 cyclic§ | 0.86 | <0.001 | 0.77 | <0.001 |
| Fibrinogen B 36–52§ | 0.84 | <0.001 | 0.73 | <0.001 |
| Fibrinogen B 54–72§ | 0.83 | <0.001 | 0.74 | <0.001 |
| Fibrinogen B 246–267 | 0.78 | <0.001 | 0.72 | <0.001 |
| Fibronectin 1029–1042 | 0.64 | <0.001 | 0.58 | 0.003 |
| Filaggrin 48–65 cyclic§ | 0.81 | <0.001 | 0.71 | <0.001 |
| Vimentin 1–16 | 0.81 | <0.001 | 0.70 | <0.001 |
| Vimentin 58–77 cyclic§ | 0.84 | <0.001 | 0.76 | <0.001 |
*
P-value calculated using Spearman’s correlation coefficient.
**
The following non-citrullinated proteins/peptides were significantly correlated with DNA-MPO and DNA-NE after adjusting for ever-smoking: Apolipoprotein A1, Fibrinogen, Histone 2A, Apolipoprotein A1 231–248, Enolase 1A 5–21, Fibrinogen A 582–599, Fibrinogen A 616–635 cyclic, Fibrinogen B 54–72 and Filaggrin 48–65 cyclic.
§
Indicates the citrullinated proteins/peptides that remained significantly correlated with DNA-MPO and DNA-NE (p≤0.002) after adjusting for ever-smoking.