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. Author manuscript; available in PMC: 2018 Sep 19.
Published in final edited form as: Nat Cell Biol. 2018 Mar 19;20(4):393–399. doi: 10.1038/s41556-018-0068-5

Figure 4. EFF-1 promotes phagosome sealing.

Figure 4

(a) TSC distal process incompletely engulfed by hyp10 cell. Arrow, phagosome opening. n=15 biologically independent animals with similar results. (b). Electron micrograph of eff-1(ns634) L1 showing open phagosome. Area not pseudocolored, cuticle. n=1 animal. (c) Enrichment of open phagosome marker (arrow) around TSC fragment. n=10 biologically independent animals with similar results. (d–f) FRAP experiment in eff-1(ns634); cup-2(ar506); ssGFP: green, secreted GFP; magenta, TSC; inset, secreted GFP (green) surrounding TSC in phagosome space. (d) before, (e) immediately after, (f) 30 minutes after photobleaching. n=11 biologically independent animals with similar results. (g) Quantification of a different FRAP experiment. n=19 biologically independent animals with similar results. (h) EFF-1(G316E) cannot rescue eff-1(ns634) distal fragment clearance defect. n=sample sizes for statistics are as follows, Line 1: n=32 (non-transgenic), n=32 (transgenic); Line 2: n=36 (non-transgenic), n=47 (transgenic); Line 3: n=47 (non-transgenic), n=43 (transgenic). (i–l) Co-localization of EFF-1 (arrowheads) and phagosome opening (arrow). n=4 biologically independent animals with similar results. (m,n) Models of EFF-1 (green) function in cell-cell fusion and phagosome sealing, respectively. Data are mean +/− s.e.m. Statistics: two-tailed unpaired t-test. Individual p values: see Supplementary Table 2. n.s., not significant (p>0.05). Numbers inside bars, total animals scored per genotype. 3 independent transgenic lines were scored for rescue experiment. FRAP experiments were repeated 38 times. Scale bars: 5 μm.