Table 1.
Repeatability and reproducibility of developed method and retention time, linearity, limit of detection (LOD) and limit of quantification (LOQ), % recovery of developed method
| Standards | Interday variability | RSD (%) | Intraday variability | RSD % | Retention time | Linearity range (mg mL−1) | Regression equation | r2 | LOD (µg mL−1) | LOQ (µg mL−1) | % Recovery |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Oxalic acid | 0.173 | 0.333 | 0.141 | 0.709 | 0.63 | 0.018–0.5 | y = 0.176x − 0.022 | 0.997 | 2.0 | 6.0 | 98 |
| Tartaric acid | 0.256 | 1.129 | 0.208 | 0.481 | 0.72 | 0.018–0.5 | y = 0.039x + 0.003 | 0.997 | 6.0 | 20.0 | 95 |
| Ascorbic acid | 0.267 | 0.216 | 0.210 | 0.476 | 0.99 | 0.018–0.5 | y = 5.766x − 0.018 | 0.998 | 12.0 | 40.0 | 109 |
| Malic acid | 0.730 | 0.000 | 0.702 | 0.142 | 1.40 | 0.018–0.5 | y = 30.37x + 0.021 | 0.998 | 12.0 | 40.0 | 100 |
| Citric acid | 0.242 | 0.239 | 0.178 | 0.858 | 1.71 | 0.018–0.5 | y = 0.501x − 0.014 | 0.985 | 3.0 | 10.0 | 113 |
| Succinic acid | 0.121 | 0.476 | 0.120 | 1.941 | 2.14 | 0.050–0.3 | y = 0.119x − 0.001 | 0.998 | 1.5 | 5.0 | 117 |
RSD Relative standard deviation, LOD limit of detection, LOQ limit of quantification