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. 2018 Mar 23;8:91. doi: 10.3389/fcimb.2018.00091

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Copyright © 2018 Hayashi, Kubo, Izumida, Takahashi, Kido, Sato, Yamaya, Nishimura, Nakayama and Matsuyama.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expressions of HA after infection of IAV [A/WSN/1933(H1N1)] in various human cell lines. Human cell lines (1 × 10⁵ cells) of various origins (HT1080, fibrosarcoma; HeLa, cervical epithelial carcinoma; A549, lung adenocarcinoma; Caco-2, colon adenocarcinoma; TE671, rhabdomyosarcoma; U937, monocyte-like histiocytic lymphoma; H292, lung mucoepidermoid carcinoma; 293T, human embryonic kidney; A431, epidermoid carcinoma; Capan-2, pancreatic adenocarcinoma; Huh7, hepatocellular carcinoma; and NB9, neuroblastoma) were plated in a 24-well plate and inoculated with IAV [A/WSN/1933(H1N1)] (MOI = 0.1). The cell lysates were prepared 48 h after IAV infection, separated electrophoretically, and subjected to western blotting with a specific antibody directed against IAV HA to estimate the amount of viral proliferation in the cells. The 65-kDa precursor IAV HA₀ protein is indicated with arrow.