Table 1.

Primer sequences used for RT-PCR.

Genes	Forward	Reverse
Enterokinase
For all variants	GCACCTGATGGCCACTTAAT	CCAGTCACTGCTGACGAGAG
To discriminate among variantsa	GAGTCATGAAGCCAGAGCGACATTTA	TGTCTTCGTCAGAACCATCTGGACA
TMPRSS2b	TAACTGGTGTGATGGCGTGT	CCGCTGTCATCCACTATTCC
TMPRSS4	CTGAACAGCCTCGATGTCAA	CAAGGGACAGTCCAGCTCTC
HATc	TCACCAGCTACACASGAATACAG	GAAATTTCATGACAACATCCGC
PRSS1d	CCACCCCCAATACGACAGGAA	TAGTCGGCGCCAGAGCTCGC
PRSS2d	CCACCCCAAATACAACAGCCG	GGGTAGTCGGCACCAGAACTCAG
PRSS3e	CGCCACCCTAAATACAACAGGGA	TGGGTAGTCAGCACCAAAGCTCAG
β-Actinf	ACTGGGACGACATGGAGAAA	GGGGTGTTGAAGGTCTCAAA

The cDNAs were amplified in 45 cycles (10 s denaturation at 98°C, 10 s annealing at 60°C, and 30 s extension at 70°C) except for

^aannealing temperature: 66°C.

^bannealing temperature: 63°C.

^cannealing temperature: 55°C.

^dannealing temperature: 62°C.

^eannealing temperature: 58°C.

^famplified in 40 cycles.