Table 2.
Primer sequences used for cloning cDNAs.
| Region | Forward | Reverse |
|---|---|---|
| Enterokinase | ||
| N-terminal halfa | ATGGGGTCGAAAAGAGGCATATCTTCTAGG | CTCAAAAGGTCCTCCACAGTCCGTAGG |
| C-terminal half | CCTACGGACTGTGGAGGACCTT | GAGTAGAATGGGAAAATAATGCGAC |
| TMPRSS2b | CAAGATGGCTTTGAACTCAGGGT | AGGACGAAGACCATGTGGATTAGC |
| TMPRSS4 | AGCATGTTACAGGATCCTGACAGTGATCAACCTC | AGCATTACAGCTCAGCCTTCCAGA |
| HAT | AAAATGTATAGGCCAGCACGTG | ACTTGTTGCACTAGATCCCAGTTT |
| PRSS1 | CCACCATGAATCCACTCCTGAT | GAGACTGAAGAGATACTGGGGGC |
| PRSS2 | CCACCATGAATCTACTTCTGATCCT | GACCAGGGGCTTTAGCTGTTGG |
| PRSS3 | CCACCATGAATCCATTCCTGAT | GAGACTGCAGAGGGACCGGG |
The cDNAs were amplified in 45 cycles (10 s denaturation at 98°C, 10 s annealing at 60°C, and 2 min extension at 70°C) except for
a
annealing temperature: 65°C.
b
annealing temperature: 58°C.