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. 2018 Mar 23;9:493. doi: 10.3389/fimmu.2018.00493

Figure 5.

Figure 5

Background binding of fluorescent beads to human peripheral blood mononuclear cells (PBMC). (A) Flow cytometric analyses demonstrating the effects of blocking agents in reducing the background binding of the SA-Blue 1.1 µm and A-Red 0.8 µm beads to cells. Top panels: non-specific binding to total PBMC; Bottom panels: background among B cells. (B) Analyses of FRα-coated A-Red 0.8 µm, SA-Blue 1.1 µm, and LumAvidin 5.6 µm beads incubated with PBMCs (to establish the background staining of the beads), or with Sp2/0-MOv18 cells (to establish binding to antigen-reactive positive cells).