Figure 1.

(A) Scheme of the possible sites where interactions between hyaluronan (HA) and lymphatic capillaries take place. The HA receptor such as LYVE-1, CD44, or RHAMM is located on both the luminal and basal surfaces of the cells. (B) The orthogonal functionalized surface used in these experiments was passivated with polyethylene glycol, which was further modified with cyclic RGD ligand (light blue tetrahedron). The nanostructures had a particle diameter of d_p = 10 nm, and were functionalized with HA oligosaccharides [turquoise, radius of gyration r_g = 7.1 nm (Kuehl et al., 2016)]. The hexagonal packed particles had variable interparticle spacings (s). (C) SEM pictures of the nanostructured surfaces with different interparticle distances. Scale bar corresponds to 100 nm.