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. 2018 Mar 23;6:56. doi: 10.3389/fchem.2018.00056

Table 1.

Antioxidant activity of synthesized compounds 20a-ab by DPPH radical scavenging assay and FRAP assay.

graphic file with name fchem-06-00056-i0001.jpg
S. No. Compound No. R1 R2 R3 R4 R5 Antioxidant activitya
FRAP assayb (C0.5FRAP μM) DPPH assayc IC50 (μmg/mL)
1 20a H H H H H 611.5 ± 23.2 10.20 ± 0.08
2 20b OCH3 H H H H 686.4 ± 30.8 6.89 ± 0.07
3 20c H H Cl H H 467.8 ± 22.4 28.80 ± 0.60
4 20d F H Cl H H 732.7 ± 41.6 45.21 ± 0.92
5 20e Cl H Cl H H >1000 92.20 ± 1.54
6 20f Cl Cl Cl H H >1000 56.60 ± 1.12
7 20g Br H Cl H H 798.6 ± 32.5 61.23 ± 1.23
8 20h CH3 H Cl H H 821.9 ± 38.7 44.83 ± 0.81
9 20i OCH3 H Cl H H 648.2 ± 29.5 34.94 ± 0.73
10 20j F H CH3 H H 502.6 ± 18.2 34.41 ± 0.70
11 20k Cl Cl CH3 H H 652.5 ± 30.6 43.58 ± 0.92
12 20l OCH3 H CH3 H H 916.8 ± 21.4 43.80 ± 0.85
13 20m H H CH3 H Br 536.7 ± 21.4 24.38 ± 0.46
14 20n F H CH3 H Br 482.5 ± 35.5 16.86 ± 0.72
15 20o Cl Cl CH3 H Br 641.6 ± 28.7 22.48 ± 0.64
16 20p Br H CH3 H Br >1000 44.32 ± 0.45
17 20q CH3 H CH3 H Br >1000 36.24 ± 0.27
18 20r NO2 H CH3 H H 328.6 ± 25.8 12.23 ± 0.05
19 20s NO2 H H H H 618.4 ± 23.9 21.27 ± 0.28
20 20t H H NO2 H H 638.2 ± 32.6 4.74 ± 0.08
21 20u F H NO2 H H 424.5 ± 19.7 32.11 ± 0.52
22 20v OCH3 H NO2 H H >1000 43.58 ± 0.92
23 20w H H H NO2 H 597.4 ± 26.4 12.53 ± 0.09
24 20x F H H NO2 H 628.6 ± 32.4 10.18 ± 0.10
25 20y Cl H H NO2 H 708.2 ± 27.1 19.76 ± 0.35
26 20z Cl Cl H NO2 H 437.6 ± 39.4 28.81 ± 0.67
27 20aa Br H H NO2 H 518.6 ± 17.6 28.37 ± 0.16
28 20ab OCH3 H H NO2 H >1000 43.60 ± 0.74
29 Ascorbic acid — — — — — — 4.57
30 BHT — — — — — 546.0 ± 13.6 —
a

Results are expressed as a mean ± standard deviation (n = 3). bDPPH radical scavenging activities are expressed as IC50 concentrations of the compounds (μg/mL) required to inhibit 50% of the radicals and the maximum inhibition values and Positive control for DPPH assay=Ascorbic acid; cPositive control for FRAP assay=BHT. The bold value indicates promising antioxidant compounds.