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. 2018 Mar 23;9:252. doi: 10.3389/fphar.2018.00252

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Copyright © 2018 Yang, Chen, Wang, Deng, Huang, Ma, Song, Du, Huang, Wen, Ren and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kurarinone dose-dependently provoked A549 cell apoptosis in vitro. (A) A549 cells were treated with 0, 5, 10 μg/mL of kurarinone and 25 μg/mL of CDDP for 24 h, after which the cells were photographed under a phase contrast microscope. Then, the cells were fixed and incubated with Hoechst 33258 for 30 min, after which the cells were photographed under a fluorescence microscope. (B) Cell cycle distribution was observed by FACS. The result showed that kurarinone induced cell death in A549 cells in a dose-dependent manner. (C) The data are shown as the means ± SD of three independent experiments. ^∗P < 0.05 compared to control, ^∗∗P < 0.01 compared to control, ^∗∗∗P < 0.001, compared to control.