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. 2018 Mar 29;8:5407. doi: 10.1038/s41598-018-23602-0

Figure 3.

Figure 3

SIRT6 is critical for damage-induced telomere movement. (a) MEF WT cells expressing the KR-TRF1 telomere label were imaged using the method shown in Fig. 1a. Damage was induced using a 559 nm laser light full-power scan 36 hr after transfection, and the average MSD was calculated. p < 0.001. (b) MEF SIRT6 KO cells expressing the KR-TRF1 telomere label were imaged using the method shown in Fig. 1a. Damage was induced using a 559 nm laser light full-power scan, and average MSD was calculated. (c,d) KR-TRF1 labeled/damaged telomeres in MEF SIRT6 KO cells transfected with GFP-SIRT6 or a GFP-vector were imaged directly after damage induction using a 559 nm laser light full-power scan, and average MSD was calculated. p < 0.001. (e) WB (left) shows the expression of GFP-SIRT6 and GFP-H133Y in MEF SIRT6 KO cells. KR-TRF1 labeled/damaged telomeres in MEF SIRT6 KO cells transfected with GFP-SIRT6 or GFP-H133Y were imaged directly after damage induction using a 559 nm laser light full-power scan, and average MSD was calculated. One hundred telomeres were used for analysis and three independent experiments were done in this analysis.