Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 29;9:1285. doi: 10.1038/s41467-018-03588-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — USP52 promotes ASF1A stabilization. a MCF-7 cells stably expressing different sets of USP52 shRNAs were collected for western blotting and qRT-PCR analysis. Each bar represents the mean ± S.D. for biological triplicate experiments. ^**P < 0.01, one-way analysis of variance (ANOVA). b Experiments analogous to a were performed in HeLa cells. Each bar represents the mean ± S.D. for biological triplicate experiments. ^**P < 0.01, one-way ANOVA. c MCF-7 cells were transfected with control siRNA or USP7 siRNA. Cellular extracts were prepared and analyzed by western blotting. d MCF-7 cells stably expressing shRNA targeting 3′UTR of USP52 were transfected with control vector or Myc-USP52 and cellular lysates were collected for western blotting analysis with antibodies against the indicated proteins. e MCF-7 cells were transfected with different amounts of wild-type USP52, USP52 lacking UCH domain (USP52/∆UCH), or USP52 lacking WD40 repeat domain (USP52/∆WD40), and cellular lysates were collected for western blotting analysis with antibodies against the indicated proteins. f MCF-7 cells stably expressing different sets of USP52 shRNAs were treated with proteasome inhibitor MG132 (10 μM) or DMSO. Cellular extracts were prepared and analyzed by western blotting. g MCF-7 cells transfected with control siRNA or USP52 siRNA were treated with cycloheximide (CHX) and harvested at the indicated time followed by western blotting analysis (upper panel). MCF-7 cells stably expressing control vector or FLAG-USP52 were treated with CHX and harvested at the indicated time followed by western blotting analysis (lower panel). h HeLa cells synchronized by double-thymidine block were released and cellular extracts were collected for western blotting analysis with antibodies against the indicated proteins. Representative cell cycle profiles are shown. i HeLa cells synchronized by double-thymidine block were released and cellular extracts were collected for co-immunoprecipitation analysis of the association of ASF1A with USP52. j Western blotting analysis of the expression of ASF1A and USP52 in multiple cell lines. The intensity of each band was quantified by densitometry with Image J software with β-actin as a normalizer. The correlation coefficient and P-values are shown