Table 1.
HLA-mismatched haematopoietic stem cell transplantation (HSCT) pairs of study cohort 1 (N = 261 sibling pairs) analysed by Immunochip single nucleotide polymorphism (SNP) array.
| Pair id | Clinical HLA typing | Imputation of HLA-A,-B,-C,-DRB1,-DQA1,-DQB1 and -DPB1 alleles | Genotyping of 5137 SNPs within 4 Mbp region of MHC | Total match | GvHD grading | ||
|---|---|---|---|---|---|---|---|
| HLA-A, -B, -DRB1 match | Mismatched HLA allele | Mismatched HLA allele | SNP mismatching in MHC | Acute | Chronic | ||
| 2329 | 5/6 | DRB1, DQB1 | DRB1, DQB1, DQA1, DPB1 | C4 to DPB1 | 11/14 | na | na |
| 3205 | 5/6 | A | A, DPB1 | A, DPB1 | 12/14 | 2 | limited |
| 4426 | 5/6 | A | A, DPB1 | A, DPB1 | 12/14 | 0 | extensive |
| 4658 | 5/6 | A | A | A | 13/14 | 2 | no |
| 4754 | 5/6 | B | B | C4 to HLA-E, telomeric to A | 12/14 | 0 | no |
| 5366 | 5/6 | B,C | B,C, DPB1 | B to C, DPB1 | 11/14 | na | na |
| 1812 | 6/6 | — | DPB1 | DPB1 | 13/14 | 0 | na |
| 3450 | 6/6 | — | DPB1 | DPB1 | 13/14 | 0 | no |
| 4152 | 6/6 | — | DPB1 | DPB1 | 13/14 | 0 | no |
| 5236 | 6/6 | — | DPB1 | DPB1 | 13/14 | 0 | no |
| 2934 | 6/6 | — | — | centromeric to DPB1 | 14/14 | 1 | extensive |
| 3446 | 6/6 | — | — | centromeric to DPB1 | 14/14 | 1 | limited |
| 4706 | 6/6 | — | — | centromeric to DPB1 | 14/14 | 0 | limited |
| 3803 | 6/6 | — | — | telomeric to A | 14/14 | 0 | extensive |
| 4205 | 6/6 | — | — | non-HLA regions | 14/14 | 3 | limited |
Six pairs were known to have one mismatched allele at HLA-A, -B or -DRB1 gene based on clinical HLA typing prior to HSCT. Four additional pairs were revealed to be mismatched by HLA allele imputation based on SNP data. Disparity outside the classical HLA genes in five pairs was uncovered by comparison of 5137 SNPs in the major histocompatibility complex region (MHC). HLA = human leukocyte antigen; SNP = single nucleotide polymorphism; MHC = major histocompatibility complex; C4 = complement component 4; GvHD = graft versus host disease.