Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 29;8:5405. doi: 10.1038/s41598-018-23731-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fractionation assay and WB analysis of RPA1 bands after IR. (A) Scheme showing the primary steps of the fractionation assay. The names indicated in red represent the fractions used in WB analysis. (B) The GAPDH protein was used as an internal control for the soluble protein fraction (SFI and II). The H3 protein was used as an internal control for the DNA-bound protein fraction (DBP). Western blot membranes were cropped to avoid cross-reactions between secondary antibodies during the revealing process. (C and D) Molecular weight migration profile of RPA1 in total, soluble protein fraction (SFI and II) and DNA bound protein (DBP) extracts after IR treatment. Arrow in panel D indicates a region where a lane was excluded from the gel, but all samples were analyzed in an unique gel.