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. 2018 Mar 29;8:5405. doi: 10.1038/s41598-018-23731-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Recruitment of Homologous Recombination (HR) repair proteins to DNA after IR. (A) WB analysis of DNA-bound proteins (DBP) derived from the fractionation assay after IR treatment. The H3 protein was used as an internal control. (B) A WB quantification of the Exo1 relative intensity after IR treatment. (C) A WB quantification of the RPA1 relative intensity after IR treatment. (D) A WB quantification of the γH2A relative intensity after IR treatment. (E) WB quantification of the RAD51 relative intensity after IR treatment. The data represent the average of three independent experiments and the error bars represent the standard deviations. (F) A sixth-degree polynomial regression of the WB quantifications. Non-parametric estimation curves and R2 factors are presented. Notably, the Western blot membranes were cropped to avoid cross-reactions between secondary antibodies during the revealing process.