Figure 6.

tPA-induced lysis of platelet-containing fibrin clots. Fibrinogen (6 µM) supplemented with 0.5 μM plasminogen, 2.5 mM CaCl₂, 10 μM ADP and in the indicated cases, with murine platelets and modulator additives was clotted with 16 nM thrombin for 60 min. Lysis was initiated by addition of 50 µg/ml tPA to the clot surface and light absorbance at 340 nm was measured. (a) Representative lysis curves of tPA-induced lysis in clots containing ROS and platelets isolated from wild type (WT) and CypD^−/− (KO) mice. The presented turbidity values are the measured absorbance values normalised for the maximal absorbance for each sample (n = 4, every 10^th measurement point is shown, bars represent SE values). The time to reach 50% (t50) and 10% (t10) of the initial turbidity was calculated from the lysis curves of clots containing (b) 1 × 10⁵/µl or (c) 2 × 10⁵/µl platelets and presented in relative units: the mean value of the t50 and t10 for platelet-free fibrin (no plt) in each independent set of measurement was considered to be 1. Mean of n = 7–8 replicates and SE values are shown. Asterisks indicate significant differences at P < 0.05 according to the post-hoc Dunn test performed after Kruskal-Wallis test for the respective triplets of data. *a: P = 0.0241, *b: P = 0.0241, *c: P = 0.0154, *d: P = 0.0021, *e: P = 0.0034, *f: P = 0.0009 *g: P = 0.0184, *h: P = 0.0239.